Proteomics Laboratory - DBSV - Varese

Within the laboratory, activities include the purification of total protein extracts, enrichment of peptides containing post-translational modifications, one- and two-dimensional electrophoretic and chromatographic separations, image analysis of 2D maps, western blotting, and protein identification and quantification by mass spectrometry coupled with bioinformatic analysis.

Equipment

• Proteomics platform based on the 2DE (two-dimensional electrophoresis) approach: IEF separation (IPGphor, GE Healthcare, and PROTEAN® i12™ IEF system, Bio-Rad), SDS-PAGE electrophoretic separation (PROTEAN II XL cells for six large gels, Mini-PROTEAN cell, Bio-Rad), image analysis (ImageMaster, PDQuest)
• Western blot system (for mini and large gels, Bio-Rad) with Lycor Odyssey imaging system
• LXQ linear ion trap (Thermo Fisher): linear ion trap mass spectrometer with ESI source. The instrument is equipped with an HPLC system (MS Pump Plus, Thermo Fisher) and an autosampler (large-scale equipment)*
• Cluster consisting of 12 CPUs dedicated to proteomic data analysis
• Bioworks software for protein identification; in-house bioinformatic analysis tools 
   

Head of the laboratory 
Prof. Candida Vannini

Technical contact 
Dr Milena Marsoni

Access conditions 
Access to the laboratory is permitted to interested University staff, subject to prior contact with the technical officer in charge. 
*Access to large-scale equipment is regulated according to the procedures set out in the University Regulations on Large-Scale Equipment (PDF attached).