Protein Biochemistry Laboratory ‘The Protein Factory 2.0’ - DBSV - Varese

The laboratory carries out molecular biology activities (DNA extraction and manipulation, PCR, transformation, transfection), microbial and animal cell cultures; protein purification, detection, and characterisation: chromatographies, SDS-PAGE and Western blot, immunofluorescence, immunoprecipitation, spectrophotometry, fluorimetry, circular dichroism, enzymatic assays, steady-state and pre-steady-state kinetics; computational bioinformatics approaches (molecular dynamics, molecular docking, evolutionary conservation analysis); quantitative analysis of metabolites by HPLC.

The equipment

• Benchtop CO2 incubator with integrated shaker, 170 L, S41i model (New Brunswick™, Eppendorf)
• UV-Vis spectrophotometers V-630 model (Jasco)
• FP-750 series fluorimeters (Jasco)
• J-815 circular dichroism model (Jasco)
• epMotion 5075 system for liquid handling (Eppendorf)
• AKTA FPLC and AKTA Purifier chromatographic systems (Cytiva)
• HPLC system with AS2059 automatic sampler, photodiode array (PDA) detector MD-2010 plus and fluorescence detector FP-920 (Jasco)

The Major equipment*

• Stopped-flow spectrofluorimeter SFM-300 (Bio-Logic Science Instruments), Responsible Prof. Gianluca Molla
• HPLC system with manual injection, PDA detector MD-2015, fluorescence detector FP-2020 and refractive index detector RI-4030 for gel permeation chromatography (GPC) (Jasco), Responsible Dr Laura Caldinelli

Responsible  
Prof. Loredano Pollegioni

Reference technician  
Dr Laura Caldinelli

Access procedures  
Access to the laboratory is permitted upon authorisation from the responsible person.

*Access to the Major equipment takes place according to the procedures defined by the University Major Equipment Regulations (PDF attached).