- Assessment methods
- Learning objectives
- Delivery method
- Teaching methods
Basic knowledge of Biochemistry, Molecular biology , Microbial biotechnology, Cellular biotechnology.
The examination is organized in a written exam concerning hours class and practical lab. The final score is given in 30ies: the examination is successful with a score ≥ 18/30.
Biotechnology is a broad discipline in which biological processes, organisms and cells are exploited to develop new technologies. The goal of these lectures is to provide an overview of the fundamental principles and more deeply of the methodologies employed in industry for enzymes and proteins production. The course is also aimed to show during laboratory hours, the most updated knowledge/skills related to molecular and physiological techniques applied to the production of industrial enzymes. Students will learn state-of-the-art techniques to improve human health and environment protection.
The course is based on theoretical lessons and experimental lab activities.
Knowledge and understanding (Knowledge and know)
Acquisition of fundamental elements concerning molecular biology, physiology and microbiology applied to industrial biotechnology processes to produce enzymes and proteins
Acquisition of information (theoretical and experimental) to carry out a project for the production of industrial molecules
Applying knowledge and understanding (Skills and know how)
Ability to read, understand and critically review a scientific text related to the field (in English).
Ability to use these knowledge to evaluate the aims and/or the results.
Ability to apply the knowledge in the field of genetic, physiological and microbial biotechniques to an industrial process proposing a problem-solving approach.
TEORETICAL LESSONS (32 HOURS, 4 CFU)
- Homologous and heterologous expression: microorganisms employed, advantages and disadvantages, new and old techniques
- Metagenomics as source of new enzymes for biotechnological use
- Production of enzymes for food industry, pharmaceutical and diagnostic
- Enzymes from extremophiles: their employment in biotech companies
- Bioconversions (vitamins and steroids)
- Some examples of production of therapeutic proteins
PRACTICAL LAB (32 hours of laboratory, 2 CFU)
The practical lab will consist in lessons divided in a first introductive part followed by practical activities done directly by students in the biological lab for students situated in via Dunant, 3.
The practices will concern from the beginning to the end, about the protein expression and partial purification of an industrial enzyme in expressed Escherichia coli or other microorganisms currently used as heterologous hosts.
Slides of lessons and practical activities (introductive part and protocols): from E-learning web-site
Scientific articles from the lecturer
Books: Brown, Biotecnologie Molecolari: Principi e tecniche, Zanichelli (2nd edition)
Demain e Davies, Manual of Industrial Microbiology and Biotechnology
The course, theoretical lessons (4 CFU) and lab activities (2 CFU) will be performed using slides showed during the lesson hours. The practical activities will be held in the Experimental Biology Labs in via Dunant, 3.
During practical activities students, will be divided in small groups of 3-4, and each group will have a bench to perform experiments. Before perform experiments student will have the protocols necessary to follow the activities. During practical lab the professor and one or two assistants will be present in the lab to guarantee assistance.
The presence to the practical activities is compulsory and will be allowed absences for a maximum of 25% of total hours.