Università degli studi dell'Insubria

ANALYTICAL CHEMISTRY LAB

Degree course: 
Corso di First cycle degree in ENVIRONMENTAL AND NATURAL SCIENCES
Academyc year when starting the degree: 
2019/2020
Year: 
1
Academyc year when helding the course: 
2019/2020
Course type: 
Basic compulsory subjects
Language: 
Italian
Credits: 
6
Period: 
Second semester
Standard lectures hours: 
72
Detail of lecture’s hours: 
Lesson (24 hours), Laboratory (48 hours)
Requirements: 

There are no rules of prerequisites but it is useful to have basic notions of mathematics, (exponential notation, logarithms, equation of first and second degree).

Final Examination: 
Orale

The evaluation will consist of a written test (2h), and an oral test (30')
Written test: 2 numerical exercises on solving problems relating to the laboratory; 1 question on theory and laboratory practice. For each test 10 points will be awarded: to enter the oral exam you must obtain at least 5 points for each individual question.
The oral exam will consist of:
1) discussion of the written test
2) brief presentation of practical theoretical topics of the course
3) discussion of reports on practical laboratory exercises
The resulting evaluation will take appropriate account of written, oral and laboratory reports.

Assessment: 
Voto Finale

Acquisition of
• Analytical Chemistry laboratory ability and introduction to theory and practice of modern instrumental analytical techniques.
• Field sampling ability, including pre-treatment and conservation of analytical samples
• judgment autonomy to evaluate and critically interpret, chemical and phyical-chemical experimental data according to analyzed environment.
• adequate skills in statistical treatment and evaluation of analytical data
• “on field” teamwork ability and evaluation of referenced data.

Statistic – Simplified approach of statistic methods in Analytical Chemistry and data evaluation (3h).
Sampling – short introduction to sampling methods, conservation and chemical digestion of samples in solid and liquid phase (4h).
Chemical equilibrium and Analytical Chemistry – application of basic rules of chemical equilibrium in Analytical Chemistry: acids and basis, red-ox, precipitation and complexation equilibria, application in titration methods (6h).
Quantitative Methods – titration methods, selecting and evaluating the End Point: use of indicator, primary and secondary standard materials (3h).
Instrumental Analysis – general information, selection criteria of analytical methods, calibration curves, methods of standards addition (2h).
Basic analytical instrumentation – molecular spectroscopic techniques, introduction to chromatography (3h).
Environmental Analysis – theoretical and practical aspects in environmental analysis, use of portable instrumentation for sampling and “in situ” analysis.

Statistic – Simplified approach of statistic methods in Analytical Chemistry and data evaluation (3h).
Sampling – short introduction to sampling methods, conservation and chemical digestion of samples in solid and liquid phase (4h).
Chemical equilibrium and Analytical Chemistry – application of basic rules of chemical equilibrium in Analytical Chemistry: acids and basis, red-ox, precipitation and complexation equilibria, application in titration methods (6h).
Quantitative Methods – titration methods, selecting and evaluating the End Point: use of indicator, primary and secondary standard materials (3h).
Instrumental Analysis – general information, selection criteria of analytical methods, calibration curves, methods of standards addition (2h).
Basic analytical instrumentation – molecular spectroscopic techniques, introduction to chromatography (3h).
Environmental Analysis – theoretical and practical aspects in environmental analysis, use of portable instrumentation for sampling and “in situ” analysis.

Laboratory – basic operations in quantitative analysis: weighing, volume measure, use of quantitative glassware. Safety and behavior rules in chemistry laboratories.
The course includes a preliminary laboratory (24h) and field activities (24h): the purpose of the in-door laboratory is to provide sufficient "chemical instruments" to carry out the sequence of operations provided by the analytical protocol adopted and to evaluate the results, including simple statistical tools, evaluating the possible interaction with the lithological arrangement of the sampling area.
The laboratory provides the following experiences and field trips:

- measure of volumes; use of the technical balance and analytical balance (3h);

- acid-base titrations: HCl and NaOH (4+4 h)

- titrations by complexation: Calcium and Magnesium determination in commercial mineral waters (4h);

- red-ox titrations: Determination of dissolved oxygen (D.O.) using the Winkler method (4h);

- use of portable instrumentation (T, pH, conductivity, DO, colorimetric kits), use of portable kits for determination of alkalinity and hardness (4h);

- Field trip: Porto Ceresio (fd 4h*)

- Field trip: Gravedona (fd 8h*)

- Potentiometric titration of carbonates in natural waters (3h)

- ionic chromatography (2h)

- Field trip: Alpe Ventina – Chiareggio (SO)(fd 12h*).

* Note: field-trips could be modified or subordinated to passing educational tests and / or replaced by integrated laboratories in case of lack of adequate hospitality facilities or for serious organizational reasons

F.W. Fifield & P.J.Haines; Environmental Analytical Chemistry, Blackwell Science
D.C.Harris, Elementi di Chimica Analitica , Zanichelli
S.E.Kegley and J Andrews, “The Chemistry of Water”, University Science Book.
L.H.Keith, “Environmental Sampling and Analysis : A Practical Guide”, Lewis Publ.

Convenzionale

Interdisciplinary approach will be follow, joining practical experiences (24 hours) with theoretical lessons (24 hours) and “on field” laboratory (24 hours). Field laboratory in lacustrine and montane environment will stress the sampling problems, the analytical approach on field, data elaboration and, finally, correct analytical report.

Student reception: appointment via email