Biochemical Methodologies

Degree course: 
Corso di First cycle degree in SCIENZE BIOLOGICHE
Academyc year when starting the degree: 
2018/2019
Year: 
2
Academyc year when helding the course: 
2019/2020
Course type: 
Compulsory subjects, characteristic of the class
Credits: 
6
Period: 
Second semester
Standard lectures hours: 
56
Detail of lecture’s hours: 
Lesson (40 hours), Laboratory (16 hours)

Written test (3-4 open questions and 10-12 multiple-choice questions) concerning the theoretical basis of the biochemical techniques. The lab activity will be evaluated through a written scientific report of the experimental activities. The final score of the module is given in 30ies and is the average of the score of the written test and of the report of the practical activities. The final score of the course is also given in 30ies and is the average of the scores of each module, weighted on the number of CFU of each module: the examination is successful if a score ≥ 18/30 is gained in each module.

Assessment: 
Voto Finale

Theory (lessons, 40 hours, 5 CFU):
- Introduction
- Electrochemical techniques
- Centrifugation
- Protein purification
- Chromatographic techniques
- Electrophoretic techniques
- Spectroscopic techniques
- Enzymatic assays
- Immunological techinques
- Techniques based on radioisotopes
- Mass spectrometry

Laboratory (16 hours, 1 CFU):
The applied laboratory activity is based on the purification and structural and functional characterization of a recombinant enzyme.

Slide of the lessons (both in classes and laboratory) are available in the Elearning website.
For the laboratory part, lecture notes prepared by the teacher will be provided.
Bonaccorsi di Patti, Contestabile, Di Salvo, METODOLOGIE BIOCHIMICHE. Espressione, purificazione e caratterizzazione delle proteine, Seconda Edizione, Zanichelli, 2019
Stoppini, Bellotti, BIOCHIMICA APPLICATA, EdiSES
Maccarrone, METODOLOGIE BIOCHIMICHE E BIOMOLECOLARI, Zanichelli, 2019

Class lessons using Power Point presentations: 40 hours
Practical laboratory activities: 16 hours

Students can meet with the professor in his office by previous email appointment (luciano.piubelli@uninsubria.it)