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The University of Insubria CV
The University of Insubria CV
The University of Insubria CV The University of Insubria CV Elena Bossi
The University of Insubria CV
 

Contact data

Assistant Professor
Department of Biotechnology and Molecular Sciences
Via J.H. Dunant 3 - 21100 Varese - Italy
Tel: +39 (0)332 421318
Fax: +39 (0)332 421318
E-mail: elena.bossi@uninsubria.it
 

Biography

2000-present Structure and function studies of Na/Cl neurotransmitter transporter and Na dependent cotransporter, using electrophysiological, biochemical, and molecular biology tools, in model systems (Xenopus oocytes and cell line). http://fisio.dipbsf.uninsubria.it/lfcm/

1998-2000 Ph.D. student, Department of Structural and Functional Biology, University of Milan (now University of Insubria), Laboratory of Cellular and Molecular Physiology (Prof. Peres), 09-12/1999 Institute of Physiology University of Zurich. Structure and function studies on Na cotransporter Na/Pi type IIb by SCAM (substitute cysteine accessibility method), electrophysiology and radio-labeled methods.

1993-1998 Technical research assistant- Department of Structural and Functional Biology, University of Milan (now University of Insubria), Laboratory of Cellular and Molecular Physiology. Properties and regulation of membrane channels, transport systems and receptors (identification, relationships and role of subunits) using electrophysiological, immunochemical, biochemical, and recombinant DNA techniques, in model systems (Xenopus oocytes) and in vitro.
02-03/1997 Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University Laboratory of Prof. Dascal: biochemical analysis of expression of voltage dependent calcium channels: identification of relationship and role of Gs protein ? subunits.
08-09/1995 CNR-NCRD Fellowship in the Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Laboratory of Prof. Dascal (Joint Project CNR-NCRD): use of molecular biology techniques in studies about heterogeneity of voltage dependent calcium channels for identification and role of subunits.

1992-1993 Research Center "Servizio Ambiente" Grandate (Italy): Development of HPLC methods in drugs, food an cosmetics.

1991-1992 ECC, JRC, Environmental Institute, Life Science Division in the laboratory of Dr. E. Marafante in collaboration with IAEA, Laboratory of marine Radioactivity, Monaco. Use of primary fish cell culture for studying effects of exposition to xenobiotics on the DNA.

1990-1991 Stage at the European Commission Community (ECC), Joint Research Center (JRC) in Ispra (Italy), Environmental Institute, Radiochemical Division, in the laboratory of Prof. S. Facchetti and Dr. E. Marafante: development of method to identify DNA's adducts with HPLC-32P-Post Labeling, and other exposition parameters to genotoxic substances.
 

Research interests

 My research interests are focused on the structure-function relationships and regulation in amino acids and neurotransmitter cotransporters. We use heterologous expression of these proteins in Xenopus oocytes and cell line, coupled to electrophysiology and fluorescence microscopy to analyse structural data involved in specific aspects of the transport function. Recently we have introduced new techniques to correlate the quaternary structure of these protein to functional data. In particularly working with FRET (Fluorescence Resonance Energy Transfer) and chimeric and concatenated constructs heterologously expressed in cell line and Xenopus oocytes we want to investigate on role of oligomerization in transporter functionality and regulation .Our future goals are to use these methodologies to investigate a possible role of transporters in neurodegenerative disease. The lepidopteran intestinal amino acid transporter KAAT1 and the rat GABA transporter GAT1 are at present our main objects of study. The techniques used include molecular biology, microinjection, patch and voltage-clamp, site-specific mutagenesis, uptake measurements and immunocytochemistry, FRET.
 

Teaching experience and appointments

 Molecular Physiology (Biology)
Laboratory of Physiological Techniques (Biology of Health)
 

Representative  publications

 1. Soragna A, Bossi E , Giovannardi S, Pisani R and Peres A Functionally independent subunits in the oligomeric structure of the GABA cotransporter rGAT1. Cell. Mol. Life Sci. (2005) in Press

2. Soragna A, Bossi E, Giovannardi S, Pisani R, Peres A. Relations between substrate affinities and charge equilibration rates in therat GABA cotransporter GAT1. J Physiol. 2005 Jan 15;562(Pt 2):333-45. Epub 2004 Oct 28.

3. Soragna A, Mari SA, Pisani R, Peres A, Castagna M, Sacchi VF, Bossi E. Structural domains involved in substrate selectivity in two neutral amino acid transporters. Am J Physiol Cell Physiol. 2004 Sep;287(3):C754-61. Epub 2004 May 12.

4. Peres A, Giovannardi S, Bossi E, Fesce R. Electrophysiological insights into the mechanism of ion-coupled cotransporters. News Physiol Sci. 2004 Apr;19:80-4. Review.

5. Mari SA, Soragna A, Castagna M, Bossi E, Peres A, Sacchi VF. Aspartate 338 contributes to the cationic specificity and to driver-amino acid coupling in the insect cotransporter KAAT1. Cell Mol Life Sci. 2004 Jan;61(2):243-56.

6. Giovannardi S, Fesce R, Bossi E, Binda F, Peres A. Cl- affects the function of the GABA cotransporter rGAT1 but preserves the mutal relationship between transient and transport currents. Cell Mol Life Sci. 2003 Mar;60(3):550-6.

7. Sacchi VF, Castagna M, Mari SA, Perego C, Bossi E, Peres A. Glutamate 59 is critical for transport function of the amino acid cotransporter KAAT1. Am J Physiol Cell Physiol. 2003 Sep;285(3):C623-32. Epub 2003 May 07.

8. Fesce R, Giovannardi S, Binda F, Bossi E, Peres A. The relation between charge movement and transport-associated currents in the rat GABA cotransporter rGAT1. J Physiol. 2002 Dec 15;545(Pt 3):739-50.

9. Bossi E, Giovannardi S, Binda F, Forlani G, Peres A. Role of anion-cation interactions on the pre-steady-state currents of the rat Na(+)-Cl(-)-dependent GABA cotransporter rGAT1. J Physiol. 2002 Jun 1;541(Pt 2):343-50.

10. Binda F, Bossi E, Giovannardi S, Forlani G, Peres A. Temperature effects on the presteady-state and transport-associated currents of GABA cotransporter rGAT1. FEBS Lett. 2002 Feb 13;512(1-3):303-7.

11. Giovannardi S, Forlani G, Balestrini M, Bossi E, Tonini R, Sturani E, Peres A, Zippel R. Modulation of the inward rectifier potassium channel IRK1 by the Ras signaling pathway. J Biol Chem. 2002 Apr 5;277(14):12158-63. Epub 2002 Jan 23.

12. Forlani G, Bossi E, Ghirardelli R, Giovannardi S, Binda F, Bonadiman L, Ielmini L, Peres A. Mutation K448E in the external loop 5 of rat GABA transporter rGAT1 induces pH sensitivity and alters substrate interactions. J Physiol. 2001 Oct 15;536(Pt 2):479-94.

13. Forlani G, Bossi E, Perego C, Giovannardi S, Peres A. Three kinds of currents in the canine betaine-GABA transporter BGT-1 expressed in Xenopus laevis oocytes. Biochim Biophys Acta. 2001 Apr 23;1538(2-3):172-80.

14. Peres A, Bossi E. Effects of pH on the uncoupled, coupled and pre-steady-state currents at the amino acid transporter KAAT1 expressed in Xenopus oocytes. J Physiol. 2000 May 15; 525 Pt 1:83-9.

15. Bossi E, Vincenti S, Sacchi VF, Peres A. Simultaneous measurements of ionic currents and leucine uptake at the amino acid cotransporter KAAT1 expressed in Xenopus laevis oocytes. Biochim Biophys Acta. 2000 Jan 10;1495(1):34-9.

16. Bossi E, Sacchi VF, Peres A. Ionic selectivity of the coupled and uncoupled currents carried by the amino acid transporter KAAT1. Pflugers Arch. 1999 Nov;438(6):788-96.

17. Bossi E, Centinaio E, Castagna M, Giovannardi S, Vincenti S, Sacchi VF, Peres A. Ion binding and permeation through the lepidopteran amino acid transporter KAAT1 expressed in Xenopus oocytes. J Physiol. 1999 Mar 15;515 ( Pt 3):729-42.

18. Blumenstein Y, Ivanina T, Shistik E, Bossi E, Peres A, Dascal N. Regulation of cardiac L-type Ca2+ channel by coexpression of G(alpha s) in Xenopus oocytes. FEBS Lett. 1999 Feb 5;444(1):78-84.

19. Bossi E, Centinaio E, Moriondo A, Peres A. Ca2+-dependence of the depolarization-inducible Na+ current of Xenopus oocytes. J Cell Physiol. 1998 Feb;174(2):154-9.

20. Centinaio E, Bossi E, Peres A. Properties of the Ca(2+)-activated Cl- current of Xenopus oocytes. Cell Mol Life Sci. 1997 Jul;53(7):604-10.

 
   
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